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ZC3H13 reduced DUOX1-mediated ferroptosis in laryngeal squamous cell carcinoma cells through m6A-dependent modification 1 1 Introduction 1 2 Methods 2 2.1 Bioinformatics 2 2.2 Cell culture 2 2.3 Gene transfection 2 2.4 Quantitative real-time PCR 2 2.5 MRNA stability assay 2 2.6 MTT assay 3 2.7 Reactive oxygen species (ROS) measurement 3 2.8 Chromatin immunoprecipitation 3 2.9 Methylated RNA immunoprecipitation (MeRIP) assay 3 2.10 Intracellular Fe2 + content assay 3 2.11 Statistical analyses 3 3 Results 3 3.1 DUOX1 is lowly expressed in LSCC and functions as a tumor suppressor 3 3.2 DUOX1 silencing promotes LSCC progression and inhibits ferroptosis 3 3.3 ZC3H13 is associated with higher ferroptosis activity and ZC3H13-mediated m6A modification reduces DUOX1 mRNA stability 4 3.4 ZC3H13 regulates ferroptosis in LSCC cells by inhibiting DUOX1 expression 7 4 Discussion 7 5 Conclusion 9 Authors’ contributions 9 Funding 10 Declaration of Competing Interest 10 Data availability 10 Acknowledgments 10 References 10

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EN

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nonfiction