About this document
DLR 4: Cell Biology Methods Overview by tandarrah is a document available to read on EtoBox.
The document describes materials and methods used in a cell biology experiment. It details three key procedures: 1) passaging C2C12 cells by removing old media, adding trypsin and PBS to lift cells, making a 1:10 split, and adding fresh media for growth. 2) Using a haemocytometer to count live cells by combining a sample with trypan blue dye and examining in a grid under a microscope. 3) Staining cells with MitoView Green dye, incubating in the dark, and observing mitochondria under a fluorescent microscope
- Author
- tandarrah
- Language
- EN