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Isolation of alkaliphilic calcifying bacteria and their feasibility for enhanced CaCO~3~ precipitation in bio‐based cementitious composites by Nafeesa Shaheen; Amna Jalil; Fazal Adnan; Rao Arsalan Khushnood is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary Microbially induced calcite precipitation (MICP), secreted through biological metabolic activity, secured an imperative position in remedial measures within the construction industry subsequent to ecological, environmental and economical returns. However, this contemporary recurrent healing system is susceptible to microbial depletion in the highly alkaline cementitious environment. Therefore, researchers are probing for alkali resistant calcifying microbes. In the present study, alkaliphilic microbes were isolated from different soil sources and screened for probable CaCO~3~ precipitation. Non‐ureolytic pathway (oxidation of organic carbon) was adopted for calcite precipitation to eliminate the production of toxic ammonia. For this purpose, calcium lactate Ca(C~3~H~5~O~3~)~2~ and calcium acetate Ca(CH~3~COO)~2~ were used as CaCO~3~ precipitation precursors. The quantification protocol for precipitated CaCO~3~ was established to select potent microbial species for implementation in the alkaline cementitious systems as more than 50% of isolates were able to precipitate CaCO~3~. Results suggested 80% of potent calcifying strains isolated in this study, portrayed higher cal

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Author

Nafeesa Shaheen; Amna Jalil; Fazal Adnan; Rao Arsalan Khushnood

Publisher

Wiley

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)