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Can I read Protection from pathogenic SIV challenge using multigenic DNA vaccines on EtoBox?
Protection from pathogenic SIV challenge using multigenic DNA vaccines by Nancy L. Haigwood; Christopher C. Pierce; Michael N. Robertson; Andrew J. Watson; David C. Montefiori; Michael Rabin; John B. Lynch; LaRene Kuller; Jannelle Thompson; William R. Morton; Raoul E. Benveniste; Shiu-Lok Hu; Philip Greenberg; Sally P. Mossman is a Immunology and Microbiology article available to read on EtoBox.
What is Protection from pathogenic SIV challenge using multigenic DNA vaccines about?
To assess DNA immunization as a strategy for protecting against HIV infection in humans, we utilized SIVmne infection of Macaca fascicularis as a vaccine challenge model with moderate pathogenic potential. We compared the efficacy of DNA immunization alone and in combination with subunit protein boosts. All of the structural and regulatory genes of SIVmne clone 8 were cloned into mammalian expression vectors under the control of the CMV IE-1 promoter. Eight M. fascicularis were immunized twice with 3 mg of plasmid DNA divided between two sites; intramuscular and intradermal. Four primed macaques received a further two DNA immunizations at weeks 16 -36, while the second group of four were boosted with 250 mg recombinant gp160 plus 250 mg recombinant Gag-Pol particles formulated in MF-59 adjuvant. Half of the controls received four immunizations of vector DNA; half received two vector DNA and two adjuvant immunizations. As expected, humoral immune responses were stronger in the macaques receiving subunit boosts, but responses were sustained in both groups. Significant neutralizing antibody titers to SIVmne were detected in one of the subunit-boosted animals and in none of the DNA-onl
Who reads Protection from pathogenic SIV challenge using multigenic DNA vaccines?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Nancy L. Haigwood; Christopher C. Pierce; Michael N. Robertson; Andrew J. Watson; David C. Montefiori; Michael Rabin; John B. Lynch; LaRene Kuller; Jannelle Thompson; William R. Morton; Raoul E. Benveniste; Shiu-Lok Hu; Philip Greenberg; Sally P. Mossman
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)
- Published
- 1999
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)