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The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection by Olena Dobrovolska; Maxim Brilkov; Noelly Madeleine; Øyvind Ødegård‐Fougner; Øyvind Strømland; Stephen R. Martin; Valeria De Marco; Evangelos Christodoulou; Knut Teigen; Johan Isaksson; Jarl Underhaug; Nathalie Reuter; Reidunn B. Aalen; Rein Aasland; Øyvind Halskau is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection about?

Chromatin post‐translational modifications are thought to be important for epigenetic effects on gene expression. Methylation of histone N‐terminal tail lysine residues constitutes one of many such modifications, executed by families of histone lysine methyltransferase (HKMTase). One such protein is ASHH2 from the flowering plant Arabidopsis thaliana, equipped with the interaction domain, CW, and the HKMTase domain, SET. The CW domain of ASHH2 is a selective binder of monomethylation at lysine 4 on histone H3 (H3K4me1) and likely helps the enzyme dock correctly onto chromatin sites. The study of CW and related interaction domains has so far been emphasizing lock–key models, missing important aspects of histone‐tail CW interactions. We here present an analysis of the ASHH2 CW‐H3K4me1 complex using NMR and molecular dynamics, as well as mutation and affinity studies of flexible coils. β‐augmentation and rearrangement of coils coincide with changes in the flexibility of the complex, in particular the η1, η3 and C‐terminal coils, but also in the β1 and β2 strands and the C‐terminal part of the ligand. Furthermore, we show that mutating residues with outlier dynamic behaviour affect the

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Author

Olena Dobrovolska; Maxim Brilkov; Noelly Madeleine; Øyvind Ødegård‐Fougner; Øyvind Strømland; Stephen R. Martin; Valeria De Marco; Evangelos Christodoulou; Knut Teigen; Johan Isaksson; Jarl Underhaug; Nathalie Reuter; Reidunn B. Aalen; Rein Aasland; Øyvind Halskau

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)