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Can I read Enhanced invasion of hormone refractory prostate cancer cells through hepatocyte growth factor (HGF) induction of urokinase‐type plasminogen activator (u‐PA) on EtoBox?
Enhanced invasion of hormone refractory prostate cancer cells through hepatocyte growth factor (HGF) induction of urokinase‐type plasminogen activator (u‐PA) by Christopher L. Hall; Rachel Tsan; Gabriele Mugnai; Andrew Mazar; Robert Radinsky; Curtis A. Pettaway is a Medicine article available to read on EtoBox.
What is Enhanced invasion of hormone refractory prostate cancer cells through hepatocyte growth factor (HGF) induction of urokinase‐type plasminogen activator (u‐PA) about?
## Abstract ## BACKGROUND Increased expression of the hepatocyte growth factor (HGF) receptor (MET) is associated with high‐grade prostatic adenocarcinoma and metastasis. However, the mechanism through which MET signaling contributes to prostate cancer (CaP) metastasis remains unclear. ## METHODS Human PC‐3 CaP cells and in vivo selected, isogeneic variant cells of increasing metastatic potential (PC‐3M, PC‐3M‐Pro4, and PC‐3M‐LN4) were used to investigate the effect of HGF on CaP cell growth, protease production, and invasion. Cell‐free urokinase‐type plasminogen activator (u‐PA) expression and function following HGF treatment were analyzed by Western blot, ELISA, and casein/plasminogen zymography. In vitro invasion stimulated by HGF was measured using Matrigel‐coated invasion chambers. ## RESULTS Both mRNA and functional protein for MET were detected in each of the CaP cell lines. HGF treatment (0–40 ng/ml) weakly increase proliferation, however, HGF induced soluble u‐PA protein and activity 3‐fold in the metastatic variant cells. HGF significantly stimulated the invasion of highly metastatic PC‐3M‐LN4 cells through Matrigel and treatment with specific urokinase receptor inhibitor
Who reads Enhanced invasion of hormone refractory prostate cancer cells through hepatocyte growth factor (HGF) induction of urokinase‐type plasminogen activator (u‐PA)?
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- Author
- Christopher L. Hall; Rachel Tsan; Gabriele Mugnai; Andrew Mazar; Robert Radinsky; Curtis A. Pettaway
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)