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Differential Expression of the Chromosome 11 Mucin Genes in Colorectal Cancer by Paul A. Sylvester; Neil Myerscough; Bryan F. Warren; Ingemar Carlstedt; Anthony P. Corfield; Paul Durdey; Michael G. Thomas is a Medicine article available to read on EtoBox.

What is Differential Expression of the Chromosome 11 Mucin Genes in Colorectal Cancer about?

## Abstract The four secretory mucin genes clustered on chromosome 11, MUC2, MUC5AC, MUC5B and MUC6, were screened in 37 patients with cancers in the left hemi‐colon or rectum and 10 normal rectal controls. The mucin genes were detected by __in situ__ hybridization using oligonucleotide probes to the variable number tandem repeat (VNTR) sequences, while the proteins were stained with non‐VNTR (MUC2, MUC5AC and MUC5B) or VNTR (MUC6) antibodies. Low levels of MUC2 mRNA were detected in non‐mucinous adenocarcinomas (5/27) while a higher proportion of mucinous carcinomas (4/9) was positive. All 25 cases of adjacent normal tissue expressed MUC2 mRNA. No transcripts for MUC5AC, MUC5B or MUC 6 were detected in any of these specimens. MUC2 protein product was detected immunohistochemically in 34/36 carcinoma specimens, with no change from normal controls. There was __de novo__ expression of MUC5AC in 23/36 carcinomas. No MUC5B or MUC6 protein was detected. No difference in MUC2 and MUC5AC protein was found between mucinous and non‐mucinous carcinomas. The level of MUC2 was increased in moderately differentiated cancers compared with normal controls and decreased in the poorly differentiate

Who reads Differential Expression of the Chromosome 11 Mucin Genes in Colorectal Cancer?

Author

Paul A. Sylvester; Neil Myerscough; Bryan F. Warren; Ingemar Carlstedt; Anthony P. Corfield; Paul Durdey; Michael G. Thomas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2001

Language

EN

Field

Medicine (Health Sciences)