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Development of a “Reverse Capture” Autoantibody Microarray for Studies of Antigen-autoantibody Profiling by Shuzhen Qin; Weiliang Qiu; Joshua R. Ehrlich; Angeline S. Ferdinand; Jerome P. Richie; Michael P. O'Leary; Mei-Ling Ting Lee; Brian C.-S. Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Development of a “Reverse Capture” Autoantibody Microarray for Studies of Antigen-autoantibody Profiling about?
Diagnosing cancers based on serum profiling is a particularly attractive concept. However, the technical challenges to analysis of the serum proteome arise from the dynamic range of protein amounts. Cancer sera contain antibodies that react with a unique group of autologous cellular antigens, which affords a dramatic amplification of signal in the form of antibodies relative to the amount of the corresponding antigens. The serum autoantibody repertoire from cancer patients might, therefore, be exploited for antigen-antibody profiling. To date, studies of antigen-antibody reactivity using microarrays have relied on recombinant proteins or synthetic peptides as arrayed features. However, recombinant proteins and/or synthetic peptides may fail to accurately detect autoantibody binding due to the lack of proper PTMs. Here we describe the development and use of a "reverse capture" autoantibody microarray. Our "reverse capture" autoantibody microarray is based on the dual-antibody sandwich immunoassay platform of ELISA, which allows the antigens to be immobilized in their native configuration. As "proof-of-principle", we demonstrate its use for antigen-autoantibody profiling with sera fr
Who reads Development of a “Reverse Capture” Autoantibody Microarray for Studies of Antigen-autoantibody Profiling?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Shuzhen Qin; Weiliang Qiu; Joshua R. Ehrlich; Angeline S. Ferdinand; Jerome P. Richie; Michael P. O'Leary; Mei-Ling Ting Lee; Brian C.-S. Liu
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)