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Can I read Optimization of Amperometric Microsensors for Monitoring Choline in the Extracellular Fluid of Brain Tissue on EtoBox?
Optimization of Amperometric Microsensors for Monitoring Choline in the Extracellular Fluid of Brain Tissue by Michael G. Garguilo; Adrian C. Michael is a Chemistry article available to read on EtoBox.
What is Optimization of Amperometric Microsensors for Monitoring Choline in the Extracellular Fluid of Brain Tissue about?
Microsensors for choline with selectivity over ascorbate have been prepared by immobilizing horseradish peroxidase and choline oxidase within a cross-linkable redox polymer deposited onto lo-pm diameter carbon fiber microcylinder electrodes, ca. 300-400 /\*rn in length. To eliminate ascorbate interference, microsensors are modified by incorporating ascorbate oxidase into the sensing membrane and by applying a Nafion overlayer. When operated at 37" C at an applied potential of -0.1 V vs. Ag/AgCl, the microsensors detect 10 PM to 1 mM choline with no interference from O-400 PM ascorbate. The modified choline microsensors detect O-100 PM choline in a linear fashion (r = 0.99>, again with no ascorbate interference. At 37" C the microsensors detect 10 /.LM Ch with a signal-to-noise ratio of 12 and a response time of ca. 15 s (lo-90% of the change in signal). In order to evaluate their performance in vivo, the microsensors were implanted into the brain of a rat alongside a micropipet from which small volumes (75-250 nl) of a choline solution were injected into the tissue.
Who reads Optimization of Amperometric Microsensors for Monitoring Choline in the Extracellular Fluid of Brain Tissue?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Michael G. Garguilo; Adrian C. Michael
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1995
- Language
- EN
- Field
- Chemistry (Physical Sciences)