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This standard operating procedure describes how to perform the Limulus Amebocyte Lysate (LAL) gel clot assay to determine the amount of endotoxin present in a solution. The assay involves reconstituting LAL lysate and endotoxin standards, preparing serial dilutions of the standards, adding lysate and samples to reaction tubes, incubating at 37°C for 1 hour, and examining the tubes for gel formation to determine positive or negative reactions. Initial quality control requires verifying the labeled lysate sen

Author
Elhuseiny Mohamed
Language
EN

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