Can I read Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat ( Triticum aestivum L .) on EtoBox?

Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat ( Triticum aestivum L .) by Debbie Laudencia-Chingcuanco; Seedhabadee Ganeshan; Frank You; Brian Fowler; Ravindra Chibbar; Olin Anderson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat ( Triticum aestivum L .) about?

## Background To identify the genes involved in the development of low temperature (LT) tolerance in hexaploid wheat, we examined the global changes in expression in response to cold of the 55,052 potentially unique genes represented in the Affymetrix Wheat Genome microarray. We compared the expression of genes in winter-habit (winter Norstar and winter Manitou) and spring-habit (spring Manitou and spring Norstar)) cultivars, wherein the locus for the vernalization gene __Vrn-A1__ was swapped between the parental winter Norstar and spring Manitou in the derived near-isogenic lines winter Manitou and spring Norstar. Global expression of genes in the crowns of 3-leaf stage plants cold-acclimated at 6°C for 0, 2, 14, 21, 38, 42, 56 and 70 days was examined. ## Results Analysis of variance of gene expression separated the samples by genetic background and by the developmental stage before or after vernalization saturation was reached. Using gene-specific ANOVA we identified 12,901 genes (at __p__ < 0.001) that change in expression with respect to both genotype and the duration of cold-treatment. We examined in more detail a subset of these genes (2,771) where expression was highly infl

Who reads Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat ( Triticum aestivum L .)?

Author

Debbie Laudencia-Chingcuanco; Seedhabadee Ganeshan; Frank You; Brian Fowler; Ravindra Chibbar; Olin Anderson

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2000-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2164)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)