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Interleukin-37 contributes to the pathogenesis of gout by affecting PDZ domain-containing 1 protein through the nuclear factor-kappa B pathway by Wei Wan; Yeqing Shi; Lianmei Ji; Xiaofang Li; Xia Xu; Dongbao Zhao is a Medicine article available to read on EtoBox.

What is Interleukin-37 contributes to the pathogenesis of gout by affecting PDZ domain-containing 1 protein through the nuclear factor-kappa B pathway about?

## Objective Our objective was to explore the molecular pathogenesis of the onset of gout and the mechanism underlying the effect of interleukin (IL)-37 on PDZ domain-containing 1 (PDZK1) protein through the nuclear factor-κB signaling pathway. ## Methods Real-time PCR and western blotting were used to detect expression of PDZK1 mRNA and protein, respectively, in the HK-2 cell line. The inhibitors pyrrolidine dithiocarbamate (PDTC) and wortmannin were added to HK-2 cells stimulated by IL-37, and changes in PDZK1 protein were detected by western blotting. ## Results Based on our previous research, we used 10 μmol/L PDTC. We detected no significant change in __PDZK1__ at the mRNA level among the IL-37, PDTC+IL-37, and wortmannin+IL-37 groups. With increasing IL-37 concentration, the protein level of PDZK1 increased. After adding wortmannin, the protein level of PDZK1 increased with increasing concentration of IL-37, albeit not significantly, and the level of PDZK1 remained lower than that with IL-37 alone. After adding PDTC, the protein level of PDZK1 showed a trend to decrease with increasing concentrations of IL-37 up to 40 ng/mL. The immunofluorescence results supported the wester

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Author

Wei Wan; Yeqing Shi; Lianmei Ji; Xiaofang Li; Xia Xu; Dongbao Zhao

Publisher

SAGE Publications

Published

2020

Language

EN

Field

Medicine (Health Sciences)