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Effects of Glutamine Supply on Growth and Metabolism of Mammalian Cells in Chemostat Culture by Nienke Vriezen; Bastiaan Romein; Karel Ch. A. M. Luyben; Johannes P. van Dijken is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Effects of Glutamine Supply on Growth and Metabolism of Mammalian Cells in Chemostat Culture about?

Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology of two mammalian cell lines were studied in steady-state chemostat cultures fed with IMDM medium with 5% serum. The cell lines used were MN12, a mouse-mouse hybridoma, and SP2/0-Ag14, a mouse myeloma often used in hybridoma fusions. Cultures, grown at a fixed dilution rate of 0.03 h -1 , were fed with media containing glutamine concentrations ranging from 0.5 to 4 mmol L -1 . Biomass dry weight and cell number were linearly proportional to the glutamine concentrations fed, between 0.5 and 2 mmol L -1 , and glutamine was completely consumed by both cell lines. From this it was concluded that glutamine was the growthlimiting substrate in this concentration range and that the standard formulation of IMDM medium contains a twofold excess of glutamine. In glutamine-limited cultures, the specific rates of ammonia and alanine production were low compared to glutamine-excess cultures containing 4 mmol L -1 glutamine in

Who reads Effects of Glutamine Supply on Growth and Metabolism of Mammalian Cells in Chemostat Culture?

Author

Nienke Vriezen; Bastiaan Romein; Karel Ch. A. M. Luyben; Johannes P. van Dijken

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)