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Glucose and Amino Acid Metabolism in Neonatal Rat Skeletal Muscle in Tissue Culture by William M. Pardridge; Luiza Duducgian-Vartavarian; Delia Casanello-Ertl; Michael R. Jones; Joel D. Kopple is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The characteristics of glucose and amino acid metabolism over a 98‐hour incubation period were studied in a primary culture of neonatal rat skeletal muscle cells. The cells formed large myotubes in culture, were spontaneously highly contractile, and had cell phosphocreatine levels exceeding ATP concentrations. Medium glucose fell from 7.2±0.2 to 1.5±0.1 mM between 0 and 98 hours; intracellular glucose was readily detectable, indicating glycolysis was limited by phosphorylation, not glucose transport. Alanine levels in the medium increased from 0.06±0.01 to 0.82±0.04 mM between 0 and 48 hours and decreased to 0.72±0.04 mM by 98 hours. The period of net alanine production correlated with the rise in the cell mass action ratio of the alanine aminotransferase reaction. Cell aspartate, glutamate, and calculated oxalacetate levels were inversely related to the cell NADH/NAD^+^ ratio, as represented by the intracellular lactate/pyruvate ratio (r=0.78–0.88). The branched chain amino acids (leucine, isoleucine, valine) were actively utilized, e.g., medium leucine fell from 0.70±0.01 to 0.30±0.06 mM between 0 and 98 hours. In addition, arginine and serine consumption was observed

Who reads Glucose and Amino Acid Metabolism in Neonatal Rat Skeletal Muscle in Tissue Culture?

Author

William M. Pardridge; Luiza Duducgian-Vartavarian; Delia Casanello-Ertl; Michael R. Jones; Joel D. Kopple

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1980

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)