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A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4^+^, CD8^+^, Caspase-3 and NF-κB in mice by Dina Aboelsoued; Nagwa I. Toaleb; Sally Ibrahim; Raafat M. Shaapan; Kadria N. Abdel Megeed is a Agricultural and Biological Sciences article available to read on EtoBox.

What is A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4^+^, CD8^+^, Caspase-3 and NF-κB in mice about?

## Background __Cryptosporidium parvum__ is a protozoan parasite of medical and veterinary importance that causes neonatal diarrhea in many vertebrate hosts. In this study, we evaluated the efficacy of an affinity-purified antigen as a __C. parvum__ vaccine candidate using ileal and liver tissues of experimentally infected neonatal mice by immunohistochemical profiling and immune scoring of CD4^+^, CD8^+^, Caspase-3, and nuclear factor kappa B (NF-κB). This vaccine was prepared from the __C. parvum__ oocysts antigen using immune affinity chromatography with cyanogen bromide-activated Sepharose-4B beads. ## Methods Thirty neonatal mice were divided into three groups (10 mice/group): (1) non-immunized non-infected, (2) non-immunized infected (using gastric tubes with a single dose of 1 × 10^5^ of __C. parvum__ oocysts in 250 μl PBS solution 1 h before a meal) and (3) immunized (twice with 40 μg/kg of purified __C. parvum__ antigen at 2-week intervals and then infected with 1 × 10^5^ __C. parvum__ oocysts simultaneously with the second group). After euthanizing the animals on the 10th day, post-infection, their ileal and liver tissues were collected and prepared for immunohistochemist

Who reads A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4^+^, CD8^+^, Caspase-3 and NF-κB in mice?

Author

Dina Aboelsoued; Nagwa I. Toaleb; Sally Ibrahim; Raafat M. Shaapan; Kadria N. Abdel Megeed

Publisher

Springer Science and Business Media LLC

Published

2023

Field

Agricultural and Biological Sciences (Life Sciences)