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Can I read Protein Trans-splicing as a Protein Ligation Tool to Study Protein Structure and Function on EtoBox?
Protein Trans-splicing as a Protein Ligation Tool to Study Protein Structure and Function by A. Sesilja Aranko; Gerrit Volkmann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Protein Trans-splicing as a Protein Ligation Tool to Study Protein Structure and Function about?
## Abstract Protein __trans__-splicing (PTS) exerted by split inteins is a protein ligation reaction which enables overcoming the barriers of conventional heterologous protein production. We provide an overview of the current state-of-the-art in split intein engineering, as well as the achievements of PTS technology in the realm of protein structure-function analyses, including incorporation of natural and artificial protein modifications, controllable protein reconstitution, segmental isotope labeling and protein cyclization. We further discuss factors crucial for the successful implementation of PTS in these protein engineering approaches, and speculate on necessary future endeavours to make PTS a universally applicable protein ligation tool.
Who reads Protein Trans-splicing as a Protein Ligation Tool to Study Protein Structure and Function?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- A. Sesilja Aranko; Gerrit Volkmann
- Publisher
- Walter de Gruyter GmbH
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)