Can I read Thermodynamic Characterization of an Intermediate State of Human Growth Hormone on EtoBox?

Thermodynamic Characterization of an Intermediate State of Human Growth Hormone by Isbuel Gomez-Orellana; Bruce Varinano; Judy Miura-Fraboni; Sam Milstein; Duncan R. Paton is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Thermodynamic Characterization of an Intermediate State of Human Growth Hormone about?

## Abstract The thermal denaturation of recombinant human growth hormone (rhGH) was studied by differential scanning calorimetry and circular dichroism spectroscopy (CD). The thermal unfolding is reversible only below pH 3.5, and under these conditions a single two‐state transition was observed between 0 and 100°C. The magnitudes of the Δ__H__ and Δ__C~P~__ of this transition indicate that it corresponds to a partial unfolding of rhGH. This is also supported by CD data, which show that significant secondary structure remains after the unfolding. Above pH 3.5 the thermal denaturation is irreversible due to the aggregation of rhGH upon unfolding. This aggregation is prevented in aqueous solutions of alcohols such as __n__‐propanol, 2‐propanol, or 1,2‐propanediol (propylene glycol), which suggests that the self‐association of rhGH is caused by hydrophobic interactions. In addition, it was found that the native state of rhGH is stable in relatively high concentrations of propylene glycol (up to 45% v/v at pH 7‐8 or 30% at pH 3) and that under these conditions the thermal unfolding is cooperative and corresponds to a transition from the native state to a partially folded state, as obser

Who reads Thermodynamic Characterization of an Intermediate State of Human Growth Hormone?

Author

Isbuel Gomez-Orellana; Bruce Varinano; Judy Miura-Fraboni; Sam Milstein; Duncan R. Paton

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)