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Can I read Sequence Variant Analysis Reveals Poor Correlations in Microbial Taxonomic Abundance Between Humans and Mice After Gnotobiotic Transfer on EtoBox?
Sequence Variant Analysis Reveals Poor Correlations in Microbial Taxonomic Abundance Between Humans and Mice After Gnotobiotic Transfer by Farnaz Fouladi; Elaine M. Glenny; Emily C. Bulik-Sullivan; Matthew C. B. Tsilimigras; Michael Sioda; Stephanie A. Thomas; Yunfei Wang; Zorka Djukic; Quyen Tang; Lisa M. Tarantino; Cynthia M. Bulik; Anthony A. Fodor; Ian M. Carroll is a Environmental Science article available to read on EtoBox.
What is Sequence Variant Analysis Reveals Poor Correlations in Microbial Taxonomic Abundance Between Humans and Mice After Gnotobiotic Transfer about?
Transplanting human gut microbiotas into germ-free (GF) mice is a popular approach to disentangle cause-and-effect relationships between enteric microbes and disease. Algorithm development has enabled sequence variant (SV) identification from 16S rRNA gene sequence data. SV analyses can identify which donor taxa colonize recipient GF mice, and how SV abundance in humans is replicated in these mice. Fecal microbiotas from 8 human subjects were used to generate 77 slurries, which were transplanted into 153 GF mice. Strong correlations between fecal and slurry microbial communities were observed; however, only 42.15 ± 9.95% of SVs successfully transferred from the donor to the corresponding recipient mouse. Firmicutes had a particularly low transfer rate and SV abundance was poorly correlated between donor and recipient pairs. Our study confirms human fecal microbiotas colonize formerly GF mice, but the engrafted community only partially resembles the input human communities. Our findings emphasize the importance of reporting a standardized transfer rate and merit the exploration of other animal models or in silico tools to understand the relationships between human gut microbiotas an
Who reads Sequence Variant Analysis Reveals Poor Correlations in Microbial Taxonomic Abundance Between Humans and Mice After Gnotobiotic Transfer?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Farnaz Fouladi; Elaine M. Glenny; Emily C. Bulik-Sullivan; Matthew C. B. Tsilimigras; Michael Sioda; Stephanie A. Thomas; Yunfei Wang; Zorka Djukic; Quyen Tang; Lisa M. Tarantino; Cynthia M. Bulik; Anthony A. Fodor; Ian M. Carroll
- Publisher
- Nature Publishing Group; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1751-7362)
- Published
- 2020
- Language
- EN
- Field
- Environmental Science (Physical Sciences)