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Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean by Ronald T. Nagao; Virginia H. Goekjian; Jong Chan Hong; Joe L. Key is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean about?

The promoter region of a soybean auxin-responsive gene, GmAux28, was analyzed to identify proteinbinding DNA sequences that may be involved in regulation of expression. Using DNase I footprinting and gel mobility shift assays, multiple regions of interaction, including eight major protein-binding sites, were observed in the GmAux28 gene. Two sequence motifs, TGACGACA and TCCACGTGTC, related to as-1/Hex and G-box elements, respectively, found in several plant promoters, were identified. Four distinct A/T-rich domains were identified; such A/T-rich domains appear to modulate, but not to specify, the expression of many genes. Two new sequence motifs, delta-1 (D1) and delta-4 (D4) were also identified. D 1 and D4 share a very similar core sequence, TAGTxxCTGT and TAGTxCTGT, respectively. In gel mobility shift analyses, D1 and D4 elements exhibit a complex interaction of binding proteins. The GmAux22 promoter also contains D l-related elements which compete with the GmAux28 elements. Sequence comparisons have identified D 1/D4-1ike sequences in several other auxin-responsive genes suggesting the possible importance of D1/D4 and the respective binding proteins in the regulation of expres

Who reads Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean?

Author

Ronald T. Nagao; Virginia H. Goekjian; Jong Chan Hong; Joe L. Key

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)