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Can I read Three carbon-/oxygen-prenyltransferases responsible for furanocoumarin synthesis in Angelica dahurica on EtoBox?
Three carbon-/oxygen-prenyltransferases responsible for furanocoumarin synthesis in Angelica dahurica by Liangliang Han; Libo Zhang; Yuedong He; Lijing Liao; Junde Li; Sheng Xu; Yucheng Zhao; Xiaohong Bian; Yuanzheng Xia is a Engineering article available to read on EtoBox.
What is Three carbon-/oxygen-prenyltransferases responsible for furanocoumarin synthesis in Angelica dahurica about?
As a popular medicinal plant, Angelica dahurica has been attached to great importance in herbal medicine. Its main active compounds are furanocoumarins (FCs) including imperatorin and isoimperator which exhibit anti-oxidant, anti-tumor, and anti-virus activities. However, the enzymes catalyzing the crucial step of FCs’ biosynthesis pathway remain unidentified. This study initially screened six prenyltransferases (PTs)-coding sequences through transcriptome and gene expression profile analysis. Combined with qRT-PCR and in vitro enzyme activity verification, two C-PTs (AdPT1, 2) which convert umbelliferone to 7-demethylsuberosin (DMS), and one O-PT (AdPT3) which transforms xanthotoxol to imperatorin were identified. Among these PTs, AdPT2 also produces a trace amount of osthenol from umbelliferone. Homologous modeling, molecular docking, and site-directed mutagenesis analysis were also conducted and it is discovered that Asp301 and Asp305 in the two Asp-rich regions are vital for the activity of AdPT1. The study is the first to conduct a frontier exploration of the biosynthesis pathways of FCs in A. dahurica and will establish a foundation for the molecular mechanism of further C-PT
Who reads Three carbon-/oxygen-prenyltransferases responsible for furanocoumarin synthesis in Angelica dahurica?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Liangliang Han; Libo Zhang; Yuedong He; Lijing Liao; Junde Li; Sheng Xu; Yucheng Zhao; Xiaohong Bian; Yuanzheng Xia
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)