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Sub‐micron and Nanoscale Feature Depth Modulates Alignment of Stromal Fibroblasts and Corneal Epithelial Cells in Serum‐rich and Serum‐free Media by Sarah A. Fraser; Yuk‐Hong Ting; Kelly S. Mallon; Amy E. Wendt; Christopher J. Murphy; Paul F. Nealey is a Engineering article available to read on EtoBox.

## Abstract Topographic features are generally accepted as being capable of modulating cell alignment. Of particular interest is the potential that topographic feature geometry induces cell alignment indirectly through impacting adsorbed proteins from the cell culture medium on the surface of the substrate. However, it has also been reported that micron‐scale feature depth significantly impacts the level of alignment of cellular populations on topography, despite being orders of magnitude larger than the average adsorbed protein layer (nm). In order to better determine the impact of biomimetic length scale topography and adsorbed protein interaction on cellular morphology we have systematically investigated the effect of combinations of sub‐micron to nanoscale feature depth and lateral pitch on corneal epithelial cell alignment. In addition we have used the unique properties of a serum‐free media alternative in direct comparison to serum‐rich medium to investigate the role of culture medium protein composition on cellular alignment to topographically patterned surfaces. Our observation that increasing groove depth elicited larger populations of corneal epithelial cells to align reg

Author

Sarah A. Fraser; Yuk‐Hong Ting; Kelly S. Mallon; Amy E. Wendt; Christopher J. Murphy; Paul F. Nealey

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)