Can I read Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods on EtoBox?

Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods by Hee Seung Kim; Rangan Mallik; David S. Hage is a Chemistry article available to read on EtoBox.

What is Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods about?

Recent studies with carbamazepine on human serum albumin (HSA) columns have noted an appreciable degree of non-specific binding on supports prepared by the Schiff base immobilization method. This work examines an alternative immobilization method for HSA based on Nhydroxysuccinimide (NHS)-activated silica. This support was prepared by reacting HPLC-grade silica directly with disuccinimidyl carbonate. The resulting material was compared to an HSA support prepared by the Schiff base method in terms of its activity for carbamazepine and non-specific interactions with this drug. When examined by frontal analysis, both supports gave comparable association equilibrium constants for carbamazepine interactions with HSA ((0.53-0.55) × 10 4 M -1 at 37 • C). However, columns prepared by the Schiff base method gave greater non-specific binding. These columns, as well as control columns prepared using the carbonyldiimidazole (CDI) immobilization method, were also evaluated for their non-specific binding to a variety of other solutes known to interact with HSA. From these results it was concluded that the NHS method was an attractive alternative to the Schiff base technique in the preparation of

Who reads Chromatographic analysis of carbamazepine binding to human serum albumin: II. Comparison of the Schiff base and N-hydroxysuccinimide immobilization methods?

Author

Hee Seung Kim; Rangan Mallik; David S. Hage

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)