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COL4A1 promotes the proliferation and migration of oral squamous cell carcinoma cells by binding to NID1 by Xiaobei Tian; Jian Sun; Changshun Li; Kun Zhang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Collagen type IV α1 chain (COL4A1) is a collagen protein that acts as a tumor-promoting factor in several types of cancer. However, the role and the potential mechanisms involving COL4A1 in oral squamous cell carcinoma (OSCC) remain unclear. Using reverse transcription-quantitative PCR and western blotting, the expression levels of COL4A1 and (nidogen-1) NID1 in OSCC cells were assessed. Cell Counting Kit-8, EdU staining and colony formation assays were used to evaluate cell proliferation. Cell migration and invasion were assessed using wound healing and Transwell invasion assays, respectively. The expression levels of proteins involved in epithelial-mesenchymal transition (EMT) were assessed using western blotting. In addition, the association between COL4A1 and NID1 was analyzed using TNMplot and the STRING database and verified by co-immunoprecipitation analysis. COL4A1 expression was found to be significantly increased in OSCC cells. Knockdown of COL4A1 expression decreased SCC-4 cell proliferation, migration and invasion, as well as the progression of EMT. In addition, COL4A1 was shown to be significantly positively associated with NID1 in OSCC and to bind to NID1. NID1 overex

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Author

Xiaobei Tian; Jian Sun; Changshun Li; Kun Zhang

Publisher

D.A. Spandidos

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)