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Acid Sphingomyelinase and Inhibition by Phosphate Ion: Role of Inhibition by Phosphatidyl‐ Myo ‐Inositol 3,4,5‐Triphosphate in Oligodendrocyte Cell Signaling by F. D. Testai; M. A. Landek; R. Goswami; M. Ahmed; G. Dawson is a Neuroscience article available to read on EtoBox.
What is Acid Sphingomyelinase and Inhibition by Phosphate Ion: Role of Inhibition by Phosphatidyl‐ Myo ‐Inositol 3,4,5‐Triphosphate in Oligodendrocyte Cell Signaling about?
## Abstract There is ample evidence that both acid (ASMase) and neutral (NSMase) sphingomyelinases play a role in cell death so inhibitors of either enzyme could have significant value as protectors against neurodegeneration. We used a fluorogenic sphingomyelinase substrate, 6‐hexadecanoylamino‐4‐methylumbelliferyl‐phosphorylcholine, and a [^14^C]choline‐labeled sphingomyelin substrate to screen large numbers of phosphocompounds for inhibition of ASMase in extracts of human oligodendroglioma cells (HOG) and neonatal rat oligodendrocytes. Non‐competitive inhibition was observed with inorganic phosphate and AMP, which was a more potent inhibitor of ASMase than cyclic AMP, ADP or ATP. However, other nucleotide phosphates, sugar phosphates, nucleotide sugars and glycerol phosphate did not inhibit ASMase. Our key finding was that phosphatidyl‐__myo__‐inositol 3,4,5‐triphosphate [PtdIns (3,4,5)P~3~] was a much more potent inhibitor of ASMase than lysophosphatidic acid or phosphatidyl‐__myo__‐inositol 4,5‐diphosphate [PtdIns(4,5)P~2~]. When PtdIns(3,4,5)P~3~ was added to cultured cells we observed 50% inhibition of ASMase but no inhibition of other lysosomal hydrolases. After transfection
Who reads Acid Sphingomyelinase and Inhibition by Phosphate Ion: Role of Inhibition by Phosphatidyl‐ Myo ‐Inositol 3,4,5‐Triphosphate in Oligodendrocyte Cell Signaling?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- F. D. Testai; M. A. Landek; R. Goswami; M. Ahmed; G. Dawson
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2004
- Language
- EN
- Field
- Neuroscience (Life Sciences)