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Immunomodulatory Efficacy of Nisin—a Bacterial Lantibiotic Peptide by D. Begde; S. Bundale; P. Mashitha; J. Rudra; N. Nashikkar; A. Upadhyay is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Immunomodulatory Efficacy of Nisin—a Bacterial Lantibiotic Peptide about?

## Abstract Nisin is a peptide bacteriocin, grouped under the category of lantibiotics. It is naturally produced by __Lactococcus lactis__ to eliminate other competing gram‐positive bacteria from its vicinity. Moreover under certain conditions it is reported to be effective against a broad range of gram‐negative bacteria as well. Thus, it has been widely used as a safe food preservative especially in the dairy industry. Because of its wide‐scale consumption, its effect on eukaryotic cells should be of great concern. Here we examine the immunomodulatory efficacy of nisin __in vitro__. MTT‐based cytotoxicity assay demonstrated nisin's cytotoxicity on human T‐cell lymphoma Jurkat cells, Molt‐4 cells and freshly cultured human lymphocytes at over 200 μM concentration (IC~50~225 μM). The cell death mechanism induced by nisin in all these lymphocyte types was independent of oligonucleosomal DNA fragmentation, as analyzed by agarose gel electrophoresis and comet assay. Additionally, scanning electron microscope and fluorescence microscopy demonstrated the ability of nisin to activate human PMNs __in vitro__. Nisin‐activated neutrophils extruded intact nuclear chromatin to form NETs, well

Who reads Immunomodulatory Efficacy of Nisin—a Bacterial Lantibiotic Peptide?

Author

D. Begde; S. Bundale; P. Mashitha; J. Rudra; N. Nashikkar; A. Upadhyay

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-2617)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)