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Lipid analysis of Neochloris oleoabundans by liquid state NMR by C.M. Beal; M.E. Webber; R.S. Ruoff; R.E. Hebner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Lipid analysis of Neochloris oleoabundans by liquid state NMR about?

## Abstract This study is an evaluation of liquid state NMR as a tool for analyzing the lipid composition of algal cultures used for biodiesel production. To demonstrate the viability of this approach, ^13^C NMR was used to analyze the lipid composition of intact cells of the algal species, __Neochloris oleoabundans__ (UTEX #1185). Two cultures were used in this study. One culture was “healthy” and grown in conventional media, whereas the other culture was “nitrogen‐starved” and grown in media that lacked nitrate. Triglyceride was determined to be present in both cultures by comparing the algal NMR spectra with published chemical shifts for a wide range of lipids and with a spectrum obtained from a triglyceride standard (glyceryl trioleate). In addition, it is shown that (1) the signal‐to‐noise ratio of the ∼29.5 ppm methylene peak is indicative of the lipid content and (2) the nitrogen‐starved culture contained a greater lipid content than the healthy culture, as expected. Furthermore, the nitrogen‐starved culture produced spectra that primarily contained the characteristic peaks of triglyceride (at ∼61.8 and ∼68.9 ppm), whereas the healthy culture produced spectra that contained

Who reads Lipid analysis of Neochloris oleoabundans by liquid state NMR?

Author

C.M. Beal; M.E. Webber; R.S. Ruoff; R.E. Hebner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)