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Stress-induced Transcriptional Readthrough Into Neighboring Genes Is Linked to Intron Retention by Shani Hadar; Anatoly Meller; Naseeb Saida; Reut Shalgi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Stress-induced Transcriptional Readthrough Into Neighboring Genes Is Linked to Intron Retention about?

## Summary Exposure to certain stresses leads to readthrough transcription. Using polyA-selected RNA-seq in mouse fibroblasts subjected to heat shock, oxidative, or osmotic stress, we found that readthrough transcription can proceed into proximal downstream genes, in a phenomenon previously termed “read-in.” We found that read-in genes share distinctive genomic characteristics; they are GC-rich and extremely short , with genomic features conserved in human. Using ribosome profiling, we found that read-in genes show significantly reduced translation. Strikingly, read-in genes demonstrate marked intron retention, mostly in their first introns, which could not be explained solely by their short introns and GC-richness, features often associated with intron retention. Finally, we revealed H3K36me3 enrichment upstream to read-in genes. Moreover, demarcation of exon-intron junctions by H3K36me3 was absent in read-in first introns. Our data portray a relationship between read-in and intron retention, suggesting they may have co-evolved to facilitate reduced translation of read-in genes during stress.

Who reads Stress-induced Transcriptional Readthrough Into Neighboring Genes Is Linked to Intron Retention?

Author

Shani Hadar; Anatoly Meller; Naseeb Saida; Reut Shalgi

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)