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Interleukin 8 response and oxidative stress in HepG2 cells treated with ethanol, acetaldehyde or lipopolysaccharide by Luis Gómez-Quiroz; Leticia Bucio; Verónica Souza; Carmen Escobar; Blanca Farfán; Elizabeth Hernández; Mina Konigsberg; Florencia Vargas-Vorackova; David Kershenobich; Ma.Concepción Gutiérrez-Ruiz is a Medicine article available to read on EtoBox.

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The aim of this work was to study the induction and secretion of interleukin 8 (IL-8) and some oxidative stress parameters after ethanol (EtOH), acetaldehyde (Ac) or lipopolysaccharide (LPS) treatment on HepG2 cells. Cells were treated with 50 mM EtOH, 175 &mgrM Ac or 1 &mgrg/ml of LPS. IL-8 induction and secretion were determined in the presence of the toxics, and the effect of antioxidants N-acetyl-L-cysteine and 1,1,3,3-tetramethyl-2-thiourea was evaluated. Further, the effect of adding polyclonal anti-human tumor necrosis factor alpha (TNF-alpha) and H(2)O(2) was studied, and catalase, superoxide dismutase and glutathione peroxidase activities were determined. Lipid peroxidation increased significantly only in Ac-treated cells. All toxics failed to decrease significantly the intracellular levels of reduced GSH. Catalase activity was diminished in all treatments, while other enzyme activities did not present changes. No change in peroxide production was found with any treatment. IL-8 secretion increased in Ac (41%) and in LPS (38%)-treated cells. Antioxidant and anti-TNF-alpha treatments decreased IL-8 secretion. H(2)O(2) (0.25 mM)-treated cells increased IL-8 secretion. IL-8 re

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Author

Luis Gómez-Quiroz; Leticia Bucio; Verónica Souza; Carmen Escobar; Blanca Farfán; Elizabeth Hernández; Mina Konigsberg; Florencia Vargas-Vorackova; David Kershenobich; Ma.Concepción Gutiérrez-Ruiz

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier BV; Wiley (ISSN 1386-6346)

Published

2003

Language

EN

Field

Medicine (Health Sciences)