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Comparison of Angiogenesis-related Factor Expression in Primary Tumor Cultures Under Normal and Hypoxic Growth Conditions by Jamie M Heinzman; Stacey L Brower; Jason E Bush is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background A localized hypoxic environment occurs during tumor growth necessitating an angiogenic response or tumor necrosis results. Novel cancer treatment strategies take advantage of tumor-induced vascularisation by combining standard chemotherapeutic agents with angiogenesis-inhibiting agents. This has extended the progression-free interval and prolonged survival in patients with various types of cancer. We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater under hypoxic conditions than under normoxia. ## Methods Fifty cell sources, including both immortalized cell lines and primary carcinoma cells, were incubated under normoxic conditions for 48 hours. Then, cells were either transferred to a hypoxic environment (1% O~2~) or maintained at normoxic conditions for an additional 48 hours. Cell culture media from both conditions was collected and analyzed via an ELISA-based assay to determine expression levels of 11 angiogenesis-related factors: VEGF, PDGF-AA, PDGF-AA/BB, IL-8, bFGF/FGF-2, EGF, IP-10/CXCL10, Flt-3 ligand, TGF-β1, TGF-β2, and TGF-β3. ## Results A linear correlation between normoxic and hypo

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Author

Jamie M Heinzman; Stacey L Brower; Jason E Bush

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1475-2867)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)