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Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes : Evidence for the participation of other residues by Marilyne Stuart-Audette; Yves Blouquit; Moshe Faraggi; Cécile Sicard-Roselli; Chantal Houée-Levin; Pierre Jollès is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes : Evidence for the participation of other residues about?
One‐electron oxidation of six different c‐type lysozymes from hen egg white, turkey egg white, human milk, horse milk, camel stomach and tortoise was studied by gamma‐ and pulse‐radiolysis. In the first step, one tryptophan side chain is oxidized to indolyl free radical, which is produced quantitatively. As shown already, the indolyl radical subsequently oxidizes a tyrosine side chain to the phenoxy radical in an intramolecular reaction. However this reaction is not total and its stoichiometry depends on the protein. Rate constants also vary between proteins, from 120·s^−1^ to 1000·s^−1^ at pH 7.0 and room temperature [extremes are hen and turkey egg white (120·s^−1^) and human milk (1000·s^−1^)]. In hen and turkey egg white lysozymes we show that another reactive site is the Asn103–Gly104 peptidic bond, which gets broken radiolytically. Tryptic digestion followed by HPLC separation and identification of the peptides was performed for nonirradiated and irradiated hen lysozyme. Fluorescence spectra of the peptides indicate that Trp108 and/or 111 remain oxidized and that Tyr20 and 53 give bityrosine. Tyr23 appears not to be involved in the process. Thus new features of long‐range int
Who reads Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes : Evidence for the participation of other residues?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Marilyne Stuart-Audette; Yves Blouquit; Moshe Faraggi; Cécile Sicard-Roselli; Chantal Houée-Levin; Pierre Jollès
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)