Can I read Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell on EtoBox?
Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell by Gužvić, Miodrag (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell about?
Many biological or pathological processes are driven by cells difficult to identify or isolate, i.e., rare cells. Very often, these cells have elusive biology. Therefore, their detailed characterization is of utmost importance. There are many approaches that allow analysis of few or even many targets within one class of biomacromolecules/analytes (e.g., DNA, RNA, proteins, etc.) in single cells. However, due to rarity of the cells of interest, there is a great need to comprehensively analyze multiple analytes within these cells, in other words to perform multi-omics analysis. In this chapter, I describe a method to isolate, separate, and amplify total mRNA and genomic DNA of a single cells, using whole transcriptome (WTA) and whole genome amplification (WGA). These WTA and WGA products enable simultaneous analysis of transcriptome and genome of a single cell using various downstream high-throughput approaches.
Who reads Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gužvić, Miodrag (author)
- Publisher
- Springer US
- Published
- 2024
- Language
- EN
- ISBN
- 9781071636213
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)