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The method of deriving human T‐cell clones alters the proportion of IL‐10‐producing cells by S. B. A. COHEN; L. M. C. WEBB; M. FELDMANN is a Immunology and Microbiology article available to read on EtoBox.

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It is now well documented that the mode of primary stimulation in the mouse determines the ratio of Th1‐, Th0‐ or Th2‐type T cells obtained. In this paper we determine whether driving and cloning human peripheral blood mononuclear cells (PBMC) non‐specifically with interleukin‐2 (IL‐2) and/or IL‐4 and mitogen, will differentially select T cells for IL‐10 production. The presence of IL‐2 during culture (with or without IL‐4) yielded predominantly Th1‐type clones (81% of clones with IL‐2 alone and 77% with IL‐2 + IL‐4) and a low frequency of Th0‐type clones (19% of clones with IL‐2 and 10% of clones with IL‐2 + IL‐4), whereas IL‐4 alone increased the yield of the IL‐4 producing Th0 (35%) clones. The proportions of IL‐2‐producing clones did not alter with treatment; however, the combination of IL‐2 + IL‐4 altered the proportions of IL‐10‐producing clones. 47% of IL‐2‐cultured cells and 51% of IL‐4‐cultured cells produced IL‐10 respectively, whereas only 22% of clones driven with IL‐2 + IL‐4 produced detectable levels of IL‐10. Thus in the preliminary experiments described here we have demonstrated that the cytokines used to initially drive out human T cells and maintain the T‐cell gro

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Author

S. B. A. COHEN; L. M. C. WEBB; M. FELDMANN

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0019-2805)

Published

1996

Language

EN

Field

Immunology and Microbiology (Life Sciences)