About this Medicine article
Modified House‐keeping Gene Expression in a Rat Tail Compression Loading‐induced Disc Degeneration Model by Takashi Yurube; Toru Takada; Hiroaki Hirata; Kenichiro Kakutani; Koichiro Maeno; Zhongying Zhang; Junya Yamamoto; Minoru Doita; Masahiro Kurosaka; Kotaro Nishida is a Medicine article available to read on EtoBox.
## Abstract House‐keeping genes (HKGs) are generally used as endogenous controls for molecular normalization in quantitative PCR analysis. However, whether all the so‐called HKGs are useful for intervertebral disc research is controversial. Our objective was, using a prevalidated rat tail static compression loading‐induced disc degeneration model, to clarify the feasibility of common HKGs for gene‐quantification in the nucleus pulposus cells. In real‐time RT‐PCR for five HKGs [β‐actin, β‐glucuronidase, β‐2 microglobulin, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and lactate dehydrogenase A (LDHA)], static compression at 1.3 MPa for up to 56 days demonstrated messenger RNA (mRNA) expression levels of consistent β‐2 microglobulin and GAPDH, slightly up‐regulated β‐glucuronidase, and fairly down‐regulated β‐actin and LDHA. Especially, β‐actin had a drastic suppression to 0.15‐fold in the loaded relative to unloaded discs at 7 days. In immunofluorescence, β‐actin showed a significant down‐regulation to almost undetectable levels from 28 days, while GAPDH was constantly detected throughout. β‐Actin mRNA and protein‐distribution are thought to be affected by the loading treatment
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- Author
- Takashi Yurube; Toru Takada; Hiroaki Hirata; Kenichiro Kakutani; Koichiro Maeno; Zhongying Zhang; Junya Yamamoto; Minoru Doita; Masahiro Kurosaka; Kotaro Nishida
- Publisher
- Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0736-0266)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)