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Comparison of 1-D and 2-D LC MS/MS methods for proteomic analysis of human serum by Martin Gilar; Petra Olivova; Asish B. Chakraborty; Aleksander Jaworski; Scott J. Geromanos; John C. Gebler is a Engineering article available to read on EtoBox.
What is Comparison of 1-D and 2-D LC MS/MS methods for proteomic analysis of human serum about?
## Abstract 1‐D and 2‐D LC methods were utilized for proteome analysis of undepleted human serum. Separation of peptides in 2‐D LC was performed either with strong cation exchange (SCX)‐RP chromatography or with an RP–RP 2‐D LC approach. Peptides were identified by MS/MS using a data‐independent acquisition approach. A peptide retention prediction model was used to highlight the potential false‐positive peptide identifications. When applying selected data filtration, we identified 52 proteins based on 316 peptides in serum in 1‐D LC setup. One hundred and eighty‐four proteins/1036 peptides and 142 proteins/905 peptides were identified in RP–RP and SCX‐RP 2‐D LC, respectively. The performance of both 2‐D LC methods for proteomic analysis is critically compared.
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It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Martin Gilar; Petra Olivova; Asish B. Chakraborty; Aleksander Jaworski; Scott J. Geromanos; John C. Gebler
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)
- Published
- 2009
- Language
- EN
- Field
- Engineering (Physical Sciences)