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Purification and characterisation of a novel broad spectrum anti-tumor L-glutaminase enzyme from marine Bacillus subtilis strain JK-79 by Jambulingam, Kiruthika ;Sudhakar, Swathi is a Agricultural and Biological Sciences article available to read on EtoBox.

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L-glutaminase is a therapeutic enzyme used in the treatment of acute lymphoblastic leukemia (ALL). In this study, the extracellular L-glutaminase produced by Bacillus subtilis strain JK-79 was purified to homogeneity. The purified L-glutaminase has specific activity of 937 U mg -1 with K M and V Max value of 8 × 10 -2 mM and 200 μM/ml min, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) confirmed it to have a molecular mass of 64 kDa. Further biochemical characterization revealed that the purified enzyme attained maximal activity at pH 7 and temperature 37°C. The enzyme was stable at 37°C for 60 min at physiologic-ph. The enzyme showed high specificity towards Lglutamine. The effect of metal ions on enzyme activity showed that NaCl, KCl and CaCl 2 enhanced the activity while AgCl 3 and CuCl 2 strongly inhibited the activity of the enzyme. The in-vitro antioxidant activity of the enzyme was investigated and the IC 50 value for DPPH and ABTS assay were 400 and 600 μg/ml, respectively. The purified enzyme showed cytotoxic activity against various Leukemic

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Author

Jambulingam, Kiruthika ;Sudhakar, Swathi

Publisher

Academic Journals (ISSN 1996-0808)

Published

2019

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)