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Antioxidant-biocompatible and Stable Catalase-based Gelatin–alginate Hydrogel Scaffold with Thermal Wound Healing Capability: Immobilization and Delivery Approach by Heidi Mohamed Abdel-Mageed; Amira Emad Abd El Aziz; Batoul Mohamed Abdel Raouf; Saleh Ahmed Mohamed; Dina Nada is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Hydrogel-based matrix prepared using biopolymers is a new frontier of emerging platforms for enzyme immobilization for biomedical applications. Catalase (CAT) delivery can be effective in inhibiting reactive oxygen species (ROS)-mediated prolongation of the wound healing process. In this study, to improve CAT stability for effective application, gelatin(Gel)–alginate (Alg) biocompatible hydrogel (Gel–Alg), as immobilization support, was prepared using calcium chloride as an ionic cross-linker. High entrapment efficiency of 92% was obtained with 2% Gel and 1.5% Alg. Hydrogel immobilized CAT (CAT–Gel–Alg) showed a wide range of pH from 4 to 9 and temperature stability between 20 to 60 °C, compared to free CAT. CAT–Gel–Alg kinetic parameters revealed an increased K~m~ (24.15 mM) and a decreased V~max~ (1.39 μmol H~2~O~2~/mg protein min) × 10^4^. CAT–Gel–Alg retained 52% of its original activity after 20 consecutive catalytic runs and displayed improved thermal stability with a higher t~1/2~ value (half-life of 100.43 vs. 46 min). In addition, 85% of the initial activity was maintained after 8 weeks’ storage at 4 °C. At 24 h after thermal injury, a statistically significant

Who reads Antioxidant-biocompatible and Stable Catalase-based Gelatin–alginate Hydrogel Scaffold with Thermal Wound Healing Capability: Immobilization and Delivery Approach?

Author

Heidi Mohamed Abdel-Mageed; Amira Emad Abd El Aziz; Batoul Mohamed Abdel Raouf; Saleh Ahmed Mohamed; Dina Nada

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)