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Characterization of extracellular vesicles by flow cytometry: Challenges and promises by Banat Gul; Feryal Syed; Shamim Khan; Abid Iqbal; Iftikhar Ahmad is a Engineering article available to read on EtoBox.

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The International Society for Extracellular Vesicles (ISEV) defines the extracellular vesicle (EV) as "the particles naturally released from the cell that are delimited by a lipid bilayer and cannot replicate, i.e. do not contain a functional nucleus". The size (diameter) of EVs ranges in ~30-1000 nm, with peak population at ~ 100 nm. Flow cytometry (FCM) is the most commonly used technique for analysis of EVs. However, accurate characterization, procedure standardization, instrument calibration and results interpretation/validation of EVs is confounded by their complex and variable composition, small size and substantial differences in physiological concentrations. Here, the challenges to and promises of FCM for characterization of EVs are discussed. Specifically, we systematically reviewed the pitfalls of FCM in the detection of (small) EVs and the corresponding strategies for enhancing the sensitivity and resolution of the instrument. The shortcomings and improvement in the overall FCM system are described in terms of reference material for calibration, the collection optics for fluorescence (FL), side scatter (SSC) and forward scatter (FSC) signals and fluidics. This study may

Who reads Characterization of extracellular vesicles by flow cytometry: Challenges and promises?

Author

Banat Gul; Feryal Syed; Shamim Khan; Abid Iqbal; Iftikhar Ahmad

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Engineering (Physical Sciences)