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Extraction Method for Preparing Pyridylamino Sugar Derivatives and Application to Porcine Gastric Mucus Glycoprotein Analysis by Hitoo Iwase; Ikuko Ishii-Karakasa; Tsukiko Urata; Tohru Saito; Kyoko Hotta is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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For the sensitive detection of free sugars and oligosaccharides, the use of their pyridylamino derivatives has now found general acceptance. To remove excess 2-aminopyridine from this derivative in a reaction mixture, gel filtration and ion-exchange chromatography were conducted. It was found in the present study that contaminated 2-aminopyridine could be selectively removed from the reaction mixture by adjusting the pH with saturated sodium bicarbonate at above 8.5 followed by extraction with benzene. By using this method, fewer purification steps and less time are required, with minimum loss of pyridylamino sugar derivatives.

Who reads Extraction Method for Preparing Pyridylamino Sugar Derivatives and Application to Porcine Gastric Mucus Glycoprotein Analysis?

Author

Hitoo Iwase; Ikuko Ishii-Karakasa; Tsukiko Urata; Tohru Saito; Kyoko Hotta

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)