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Carbon Dioxide Extraction of Residual Solvents in Poly(lactide-co-glycolide) Microparticles by John Herberger; Keith Murphy; Louis Munyakazi; Jon Cordia; Eric Westhaus is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Carbon Dioxide Extraction of Residual Solvents in Poly(lactide-co-glycolide) Microparticles about?

A process for the reduction of residual solvents in spray-dried poly(lactide-co-glycolide) (PLGA)-darbepoetin alfa microparticles was developed using carbon dioxide (CO(2)) as an extraction solvent. CO(2) was investigated in two phase states, liquid and gas. Detrimental effects on encapsulated protein integrity and microparticle morphology were observed with liquid CO(2) exposure. Extraction with CO(2) gas at <100 psig reduced residual solvent concentration and particle agglomeration was limited. Extraction rates and particle agglomeration increased with higher CO(2) gas pressures. The CO(2) pressures below which particles of polylactide (PLA) and PLGA microparticles significantly agglomerated were determined and the data used to develop extraction cycles. Extraction cycles were developed in which CO(2) gas pressure was increased as residual solvent concentration decreased in order to keep extraction rates high throughout the cycle. Spray dried darbepoetin alfa-PLGA microparticles were extracted with CO(2) gas and characterized for residual solvent concentration, process yield, particle size distribution, morphology, and protein integrity. The results indicated CO(2) gas may be use

Who reads Carbon Dioxide Extraction of Residual Solvents in Poly(lactide-co-glycolide) Microparticles?

Author

John Herberger; Keith Murphy; Louis Munyakazi; Jon Cordia; Eric Westhaus

Publisher

Elsevier BV

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)