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Blockade of Maitotoxin-induced Oncotic Cell Death Reveals Zeiosis by Mark Estacion; William P Schilling is a Medicine article available to read on EtoBox.

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Background: Maitotoxin (MTX) initiates cell death by sequentially activating 1) Ca 2+ influx via non-selective cation channels, 2) uptake of vital dyes via formation of large pores, and 3) release of lactate dehydrogenase, an indication of cell lysis. MTX also causes formation of membrane blebs, which dramatically dilate during the cytolysis phase. To determine the role of phospholipase C (PLC) in the cell death cascade, U73122, a specific inhibitor of PLC, and U73343, an inactive analog, were examined on MTX-induced responses in bovine aortic endothelial cells. Results: Addition of either U73122 or U73343, prior to MTX, produced a concentrationdependent inhibition of the cell death cascade (IC 50 Ϸ 1.9 and 0.66 μM, respectively) suggesting that the effect of these agents was independent of PLC. Addition of U73343 shortly after MTX, prevented or attenuated the effects of the toxin, but addition at later times had little or no effect. Time-lapse videomicroscopy showed that U73343 dramatically altered the blebbing profile of MTX-treated cells. Specifically, U73343 blocked bleb dilation and converted the initial blebbing event into "zeiosis", a type of membrane blebbing commonly assoc

Who reads Blockade of Maitotoxin-induced Oncotic Cell Death Reveals Zeiosis?

Author

Mark Estacion; William P Schilling

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6793)

Published

2002

Language

EN

Field

Medicine (Life Sciences)