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What is Optimizing hGH Expression in E. coli about?

The study investigates the high-level expression of recombinant human growth hormone (hGH) in Escherichia coli by optimizing the translation initiation region, specifically focusing on codon usage and GC content at the N-terminal region of hGH cDNA. Results indicate that reducing GC content and optimizing codon usage significantly enhance hGH expression levels compared to the wild gene expression vector. The findings underscore the importance of these modifications in improving the efficacy of recombinant p

Author

winningcastle2.0

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EN