Can I read Chondroprotective effects of a proanthocyanidin rich Amazonian genonutrient reflects direct inhibition of matrix metalloproteinases and upregulation of IGF-1 production by human chondrocytes on EtoBox?

Chondroprotective effects of a proanthocyanidin rich Amazonian genonutrient reflects direct inhibition of matrix metalloproteinases and upregulation of IGF-1 production by human chondrocytes by Mark JS Miller; Paul Bobrowski; Meenakshi Shukla; Kalpana Gupta; Tariq M Haqqi is a Medicine article available to read on EtoBox.

What is Chondroprotective effects of a proanthocyanidin rich Amazonian genonutrient reflects direct inhibition of matrix metalloproteinases and upregulation of IGF-1 production by human chondrocytes about?

## Background The Amazonian medicinal plant Sangre de grado (__Croton palanostigma__) has traditional applications for the treatment of wound healing and inflammation. We sought to characterize two extracts (progrado and zangrado) in terms of safety and oligomeric proanthocyanidin chain length. Additionally progrado was evaluated for antioxidant activity and possible chondroprotective actions. ## Methods Acute oral safety and toxicity was tested in rats according under OECD protocol number 420. The profile of proanthocyanidin oligomers was determined by HPLC and progrado's antioxidant activity quantified by the ORAC, NORAC and HORAC assays. Human cartilage explants, obtained from surgical specimens, were used to assess chondroproteciton with activity related to direct inhibitory effects on human matrix metalloproteinase (MMP, gelatinolytic) activity using synovial fluid and chondrocytes activated with IL-1β (10 ng/ml). Additionally, progrado (2–10 μg/ml) was tested for its ability to maintain optimal IGF-1 transcription and translation in cartilage explants and cultured chondrocytes. ## Results Both progrado and zangrado at doses up to 2000 mg/kg (po) displayed no evidence of toxic

Who reads Chondroprotective effects of a proanthocyanidin rich Amazonian genonutrient reflects direct inhibition of matrix metalloproteinases and upregulation of IGF-1 production by human chondrocytes?

Author

Mark JS Miller; Paul Bobrowski; Meenakshi Shukla; Kalpana Gupta; Tariq M Haqqi

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2004-; Springer Science and Business Media LLC (ISSN 1476-9255)

Published

2007

Language

EN

Field

Medicine (Life Sciences)