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Effect of Epidermal Growth Factor and Transforming Growth Factor β1 on Growth and Invasive Potentials of Newly Established Rat Bladder Carcinoma Cell Lines by Hitoshi Kawamata; Shuji Kameyama; Li Nan; Koji Kawai; Ryoichi Oyasu is a Medicine article available to read on EtoBox.

What is Effect of Epidermal Growth Factor and Transforming Growth Factor β1 on Growth and Invasive Potentials of Newly Established Rat Bladder Carcinoma Cell Lines about?

We established 5 rat bladder cell lines (MYU3L, MYU4, MYU6s. MYKU I L and MYP3). EGF stimulated DNA synthesis of all the cells in monolayer culture, regardless of the number of EGF receptors. In soft agar, only MYU3L formed colonies, and EGF enhanced their growth. However, EGF did not induce the other cells to grow in soft agar. In contrast, TGF-f3 I inhibited the growth of the cells, but a tumorigenic cell and the cells which were established from large in vivo tumors were more resistant than the others to TGF-p I. We tested the effect of growth factors on the invasive potential of MYP3 cells (nontumorigenic), MYU3L cells (tumorigenic/highly invasive but not metastatic) from newly established cell lines, and another metastatic cell line, LMC19. MYP3 expressed only a trace amount of 92-kDa gelatinase (MMP-9). whereas MYU3L expressed interstitial collagenase (MMP-I) and MMP-9, and LMC I9 expressed 72-kDa gelatinase (MMP-2) and MMP-9. The release of MMP-2 in LMC I 9 was stimulated by TGF-f3 I, but EGF had no effect on the release of any MMPs in either type of cells. These observations suggest that EGF acted as a mitogen on all the cells tested, but did not enhance the malignant pheno

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Author

Hitoshi Kawamata; Shuji Kameyama; Li Nan; Koji Kawai; Ryoichi Oyasu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1993

Language

EN

Field

Medicine (Health Sciences)