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Development of a Capillary Electrophoresis Method for the Screening of Human Urine for Multiple Drugs of Abuse by Ahmed Alnajjar; Abubakr M. Idris; Marika Multzenberg; Bruce McCord is a Chemistry article available to read on EtoBox.

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A new capillary electrophoresis (CE) method was developed and validated for the screening of human urine for nineteen drugs of abuse. In order to achieve sufficient separation, the electrolyte composition was modified using ␤-cyclodextrin (␤-CD) and organic solvents. To process each sample, a sequential injection-solid-phase extraction (SI-SPE) system was constructed. Using this device, matrix clean-up, extraction, and preconcentration of analytes were performed onto a C 18 cartridge. Optimal separation and detection were obtained using a background electrolyte consisting of 100 mM phosphate adjusted to pH 6.0, with 20 mM ␤-CD, 5% acetonitrile and 20% isopropanol. Electrokinetic injection was performed at 5 kV for 10 s, separation voltage was 25 kV and column temperature was set to 25 • C. The separation was carried out in a 67.0 cm × 50 m fused-silica capillary with UV detection at 214 nm. The combination of SI-SPE and sample stacking showed significant sensitivity enhancement with limits of detection in the range of 5-30 ng ml -1 . A validation study showed good reproducibility of both migration time (RSD = 0.003-0.088%) and peak area (RSD = 0.54-4.8%). Overall, this automated an

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Author

Ahmed Alnajjar; Abubakr M. Idris; Marika Multzenberg; Bruce McCord

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)