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RFLP mapping of the ym4 virus resistance gene in barley by A. Graner; E. Bauer is a Agricultural and Biological Sciences article available to read on EtoBox.

What is RFLP mapping of the ym4 virus resistance gene in barley about?

RFLP (restriction fragment length polymorphism) mapping of a recessive gene (ym4) conferring resistance to barley yellow mosaic and barley mild mosaic virus was performed using progeny of 86 F1 anther-derived doubled haploid lines. Two closely linked RFLP markers that flank the gene at a distance of 1.2 centiMorgans were identified. Using one of these markers (MWG10) we obtained a clear differentiation between resistant and susceptible German cultivars. An analysis of a series of unrelated barley lines with probe MWG10 did not reveal additional RFLP fragments. The use of this probe for both marker-assisted selection and the generation of a high-density map around the resistance locus is discussed.

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Author

A. Graner; E. Bauer

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)