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DNA sequence recognition by an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) by Andrew J. Hampshire; Hannah Khairallah; Abedawn I. Khalaf; Abdolrasoul H. Ebrahimabadi; Roger D. Waigh; Colin J. Suckling; Tom Brown; Keith R. Fox is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA sequence recognition by an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) about?

We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA. While the parent compound (thiazotropsin A) binds to the hexanucleotide sequence ACTAGT, changing one of the N-methylpyrrole groups to N-methylimidazole changes the preferred binding sequence to (A/T)CGCG(T/A). Experiments with DNA fragments that contain variants of this sequence suggest that the ligand can also bind, with lower affinity, to sequences which differ from this by 1 bp in any position.

Who reads DNA sequence recognition by an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B)?

Author

Andrew J. Hampshire; Hannah Khairallah; Abedawn I. Khalaf; Abdolrasoul H. Ebrahimabadi; Roger D. Waigh; Colin J. Suckling; Tom Brown; Keith R. Fox

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0960-894X)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)