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Pyruvate-Induced changes in hepatoma cell morphology and macromolecular synthesis by L. Pickart; M. Michael Thaler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Cells maintained in basal growth medium with 0.2–1.0% serum often require citric acid cycle intermediates for optimal viability. We have found that pyruvate added to minimal growth medium causes cellular flattening and formation of external processes accompanied by increaded DNA synthesis in cultured hepatoma cells (HTC cells). Cells were cultured in plalstic T‐flasks (0.5, 1.0, or 2.0 × 10^6^ cells/flask) containing 5 ml medium (90% Eagle's Basal Medium (BME) and 10% Swim's S‐77) with various concentrations of fetal calf serum (0.2,0.25, 0.5, 1.0, 2.0, 10%) and either pyruvate (50, 100, 250,500, 1,000μg/ml), or one of: dibutyryl cAMP (DBcAMP) or dibutyryl cGMP (DBcGMP) at 10^−3^, 10^−4^, or 10^−5^ M. At 44–48 hr cultures were pulsed with tritiated thymidine, uridine, or lecucine. Cells became attached to the plastic surface within 24hr. Cells in medium with 0.25 to 2.0% serum had a rounded appearance. With added pyruvate, cellular flattening, process formation, and an increased adherence to the substratum was absorbed. By 48 hr, culture without pyruvate grew in rounded clusters; with pyruvate, cells formed extensive interconnecting processes that appeared loosely attac

Who reads Pyruvate-Induced changes in hepatoma cell morphology and macromolecular synthesis?

Author

L. Pickart; M. Michael Thaler

Publisher

Wiley (John Wiley & Sons); Alan R. Liss, Inc.; Wiley (ISSN 0091-7419)

Published

1976

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)