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Ultrasensitive immunosensing of tuberculosis CFP-10 based on SPR spectroscopy by Seong Cheol Hong; Hongxia Chen; Jaewook Lee; Hye-Kyung Park; Young Sik Kim; Hyun-Chul Shin; Cheol-Min Kim; Tae Jung Park; Seok Jae Lee; Kwangnak Koh; Hwa-Jung Kim; Chulhun L. Chang; Jaebeom Lee is a Engineering article available to read on EtoBox.
What is Ultrasensitive immunosensing of tuberculosis CFP-10 based on SPR spectroscopy about?
An antigen (Ag), CFP-10, found in tissue fluids of tuberculosis (TB) patients may be an ultimate candidate for use as a sensitive TB marker with a sensing method for early simplified diagnosis of TB. In this study, chemical and optical optimizations were carried out using novel immuno-materials for establishment of a self-assembled surface plasmon resonance (SPR) optical immunosensor system for detection of CFP-10, which is valuable for pre-clinical work, prior to conduct of massive clinical observations. For creation of a simple sensing interface, a monoclonal antibody (anti-CFP-10) was immobilized directly on a gold surface, followed by blocking with cystamine. Orientation and accessibility of anti-CFP-10 were assessed by the selective binding of CFP-10. Recent results indicate that the reusability of the sensor chip adopting the cystamine method was found to be preferable to other immobilization methods. A linear relationship was well correlated between SPR angle shift and CFP concentrations in the range from 100 ng mL -1 to 1 g mL -1 . Modification of the SPR chip with antibody provides a simple experimental platform for investigation of isolated proteins under experimental con
Who reads Ultrasensitive immunosensing of tuberculosis CFP-10 based on SPR spectroscopy?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Seong Cheol Hong; Hongxia Chen; Jaewook Lee; Hye-Kyung Park; Young Sik Kim; Hyun-Chul Shin; Cheol-Min Kim; Tae Jung Park; Seok Jae Lee; Kwangnak Koh; Hwa-Jung Kim; Chulhun L. Chang; Jaebeom Lee
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)
- Published
- 2011
- Language
- EN
- Field
- Engineering (Physical Sciences)