Can I read Determination of a Cholesterol Oxide Mixture by a Single-run High-performance Liquid Chromatographic Analysis Using Benzoylation on EtoBox?
Determination of a Cholesterol Oxide Mixture by a Single-run High-performance Liquid Chromatographic Analysis Using Benzoylation by Lucie Fillion; John A. Zee; Claude Gosselin is a Chemistry article available to read on EtoBox.
What is Determination of a Cholesterol Oxide Mixture by a Single-run High-performance Liquid Chromatographic Analysis Using Benzoylation about?
A rapid, single-run high-performance liquid chromatographic method has been developed to separate and quantify benzoate esters of cholesterol oxides, providing high sensitivity via ultraviolet detection. The mobile phase was 85% isopropanol-water (v/v). Analyses of 7-ketocholesterol, cholestane-triol, epoxycholesterol, 7-hydroxycholesterol and 25-hydroxycholesterol were done using a 30 cm x 3.9 mm I.D. Novapak C,, column and a variable-wavelength ultraviolet detector (set at 230 nm). Linearity was excellent since a good correlation was observed. As low as 500 ng of cholesterol benzoate per 20 ~1 of solution can be detected by this method.
Who reads Determination of a Cholesterol Oxide Mixture by a Single-run High-performance Liquid Chromatographic Analysis Using Benzoylation?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Lucie Fillion; John A. Zee; Claude Gosselin
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1991
- Language
- EN
- Field
- Chemistry (Physical Sciences)