Can I read HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method on EtoBox?

HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method by Dr Mark J. Arends; Yvonne K. Donaldson; Edward Duvall; Andrew H. Wyllie; Colin C. Bird is a Medicine article available to read on EtoBox.

What is HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method about?

## Abstract A new type‐specific, sensitive, non‐radioactive assay is described for the detection of human papillomavirus (HPV) DNA in tissues. Sequences within the E6 gene were amplified by the polymerase chain reaction (PCR), using primer pairs which clearly distinguish HPV types, including those with close sequency homology such as 6b and 11. The amplified DNA products were identified by non‐radioactive oligonucleotide hybridization and restriction endo‐nuclease mapping, and the method was sufficiently sensitive to detect between 3 and 5 SiHa cells (each containing 1–2) copies of (HPV 16 DNA) amongst 10 000 non‐HPV‐ containing cells. Frozen and archival paraffin sections were equally acceptable substrates for the reaction. The assay was applied to frozen sections full thickness cervical epithelium from 60 cases of cervical intraepithelial neoplasia (CIN) and 24 normal cervical controls. Prevalence of HPV 16 was similar (approximately 50 per cent) in both CIN 2 and CIN 3, and in the whole series HPV 16 was almost five‐fold more common than HPV 18. Low‐risk HPV types were present in 5 per cent of CIN 1, but 0 per cent of CIN2 and CIN3 biopsies. The data emphasize the biological sim

Who reads HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method?

Author

Dr Mark J. Arends; Yvonne K. Donaldson; Edward Duvall; Andrew H. Wyllie; Colin C. Bird

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

1991

Language

EN

Field

Medicine (Health Sciences)